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Electrophoretic mobilities of a viral capsid, its capsid protein, and their relation to viral assembly

TitleElectrophoretic mobilities of a viral capsid, its capsid protein, and their relation to viral assembly
Publication TypeJournal Article
Year of Publication2014
Authors-Acosta, JR, V, Cadena-Nava, RD, Gelbart, WM, Knobler, CM, Ruiz-Garcia, J
JournalThe Journal of Physical Chemistry B
Volume118
Pagination1984–1989
Abstract

The self-assembly of many viral capsids is dominated by protein–protein electrostaticinteractions. To have a better understanding of this process, it is important to know how the protein and the capsid surface charges vary as a function of the pH and ionic strength. In this work, using phase analysis light scattering, we measured the electrophoretic mobility (EM) of the cowpea chlorotic mottle virus (CCMV), its capsid protein (CP), and a cleaved CP that lacks its basic terminus, as a function of pH and ionic strength. The EM measurements of ...

URLhttp://pubs.acs.org/doi/abs/10.1021/jp407379t
DOI10.1021/jp407379t
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