Electrophoretic mobilities of a viral capsid, its capsid protein, and their relation to viral assembly
|Title||Electrophoretic mobilities of a viral capsid, its capsid protein, and their relation to viral assembly|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||-Acosta, JR, V, Cadena-Nava, RD, Gelbart, WM, Knobler, CM, Ruiz-Garcia, J|
|Journal||The Journal of Physical Chemistry B|
The self-assembly of many viral capsids is dominated by protein–protein electrostaticinteractions. To have a better understanding of this process, it is important to know how the protein and the capsid surface charges vary as a function of the pH and ionic strength. In this work, using phase analysis light scattering, we measured the electrophoretic mobility (EM) of the cowpea chlorotic mottle virus (CCMV), its capsid protein (CP), and a cleaved CP that lacks its basic terminus, as a function of pH and ionic strength. The EM measurements of ...