Exploiting fluorescent polymers to probe the self-assembly of virus-like particles
|Title||Exploiting fluorescent polymers to probe the self-assembly of virus-like particles|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Cadena-Nava, RD, Hu, Y, Garmann, RF, Ng, B, Zelikin, AN, Knobler, CM, Gelbart, WM|
|Journal||The Journal of Physical Chemistry B|
The inside surfaces of the protein shells of many viruses are positively charged, therebyenhancing the self-assembly of capsid proteins around their (oppositely charged) RNAgenome. These proteins have been shown to organize similarly around a variety ofnonbiological, negatively charged, polymers, for example, poly (styrene sulfonate)(PSS),forming virus-like particles (VLPs). We have demonstrated recently that the VLPs formedfrom cowpea chlorotic mottle virus (CCMV) capsid protein increase in size (from T= 2 to T=...