Reconstituted plant viral capsids can release genes to mammalian cells
|Title||Reconstituted plant viral capsids can release genes to mammalian cells|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Azizgolshani, O, Garmann, RF, Cadena-Nava, R, Knobler, CM, Gelbart, WM|
The nucleocapsids of many plant viruses are significantly more robust and protective of their RNA contents than those of enveloped animal viruses. In particular, the capsid protein (CP) of the plant virus Cowpea Chlorotic Mottle Virus (CCMV) is of special interest because it has been shown to spontaneously package, with high efficiency, a large range of lengths and sequences of single-stranded RNA molecules. In this work we demonstrate that hybrid virus-like particles, assembled in vitro from CCMV CP and a heterologous RNA derived from a ...