Rescue of infectious particles from preassembled alphavirus nucleocapsid cores
|Title||Rescue of infectious particles from preassembled alphavirus nucleocapsid cores|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Snyder, JE, Azizgolshani, O, Wu, B, He, Y, Lee, ACheun, Jose, J, Suter, DM, Knobler, CM, Gelbart, WM, Kuhn, RJ|
|Journal||Journal of virology|
ABSTRACT Alphaviruses are small, spherical, enveloped, positive-sense, single-stranded, RNA viruses responsible for considerable human and animal disease. Using microinjection of preassembled cores as a tool, a system has been established to study the assembly and budding process of Sindbis virus, the type member of the alphaviruses. We demonstrate the release of infectious virus-like particles from cells expressing Sindbis virus envelope glycoproteins following microinjection of Sindbis virus nucleocapsids purified from the ...