Salt-dependent DNA-DNA spacings in intact bacteriophage $łambda$ reflect relative importance of DNA self-repulsion and bending energies
|Title||Salt-dependent DNA-DNA spacings in intact bacteriophage $łambda$ reflect relative importance of DNA self-repulsion and bending energies|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Qiu, X, Rau, DC, V Parsegian, A, Fang, LTai, Knobler, CM, Gelbart, WM|
|Journal||Physical review letters|
Using solution synchrotron x-ray scattering, we measure the variation of DNA-DNA d spacings in bacteriophage λ with mono-, di-, and polyvalent salt concentrations, for wild-type [48.5× 10 3 base pairs (bp)] and short-genome-mutant (37.8 kbp) strains. From the decrease in d spacings with increasing salt, we deduce the relative contributions of DNA self-repulsion and bending to the energetics of packaged phage genomes. We quantify the DNA-DNA interaction energies within the intact phage by combining the measured d spacings ...