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Salt-dependent DNA-DNA spacings in intact bacteriophage $łambda$ reflect relative importance of DNA self-repulsion and bending energies

TitleSalt-dependent DNA-DNA spacings in intact bacteriophage $łambda$ reflect relative importance of DNA self-repulsion and bending energies
Publication TypeJournal Article
Year of Publication2011
AuthorsQiu, X, Rau, DC, V Parsegian, A, Fang, LTai, Knobler, CM, Gelbart, WM
JournalPhysical review letters
Volume106
Pagination028102
Abstract

Using solution synchrotron x-ray scattering, we measure the variation of DNA-DNA d spacings in bacteriophage λ with mono-, di-, and polyvalent salt concentrations, for wild-type [48.5× 10 3 base pairs (bp)] and short-genome-mutant (37.8 kbp) strains. From the decrease in d spacings with increasing salt, we deduce the relative contributions of DNA self-repulsion and bending to the energetics of packaged phage genomes. We quantify the DNA-DNA interaction energies within the intact phage by combining the measured d spacings ...

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