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Visualizing large RNA molecules in solution

TitleVisualizing large RNA molecules in solution
Publication TypeJournal Article
Year of Publication2012
AuthorsGopal, A, Z Zhou, H, Knobler, CM, Gelbart, WM
JournalRna
Volume18
Pagination284–299
Abstract

Abstract Single-stranded RNAs (ssRNAs) longer than a few hundred nucleotides do nothave a unique structure in solution. Their equilibrium properties therefore reflect the average of an ensemble of structures. We use cryo-electron microscopy to image projections of individual long ssRNA molecules and characterize the anisotropy of their ensembles in solution. A flattened prolate volume is found to best represent the shapes of these ensembles. The measured sizes and anisotropies are in good agreement with ...

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