|Title||The effect of genome length on ejection forces in bacteriophage lambda|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||P. Grayson, A. Evilevitch, M. M. Inamdar, P. K. Purohit, W. M. Gelbart, C. M. Knobler and R. Phillips|
|Type of Article||Journal Article|
|Keywords||bacteriophage, DNA EJECTION, genome delivery, LamB, lambda, maltoporin, pressure|
A variety of viruses tightly pack their genetic material into protein capsids that are barely large enough to enclose the genome. In particular, in bacteriophages, forces as high as 60 pN are encountered during packaging and ejection, produced by DNA bending elasticity and self-interactions. The high forces are believed to be important for the ejection process, though the extent of their involvement is not yet clear. As a result, there is a need for quantitative models and experiments that reveal the nature of the forces relevant to DNA ejection, Here, we report measurements of the ejection forces for two different mutants of bacteriophage lambda, lambda b221cI26 and lambda cI60, which differ in genome length by similar to 30%. As expected for a force-driven ejection mechanism, the osmotic pressure at which DNA release is completely inhibited varies with the genome length: we find inhibition pressures of 15 atm and 25 atm, for the short and long genomes, respectively, values that are in agreement with our theoretical calculations. (c) 2006 Elsevier Inc. All rights reserved.