| Title | Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection |
| Publication Type | Journal Article |
| Year of Publication | 2005 |
| Authors | A. Evilevitch, J. W. Gober, M. Phillips, C. M. Knobler and W. M. Gelbart |
| Journal Title | BIOPHYSICAL JOURNAL |
| Volume | 88 |
| Issue | 1 |
| Pages | 751-756 |
| Date Published | JAN |
| Type of Article | Journal Article |
| Keywords | BACTERIOPHAGE-LAMBDA, EXPRESSION, FORCES, PHAGE, SEDIMENTATION |
| Abstract | The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage-lambda was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids. |
| URL | http://dx.doi.org/10.1529/biophysj.104.045088 |
