| Title | DNA packaging and ejection forces in bacteriophage |
| Publication Type | Journal Article |
| Year of Publication | 2001 |
| Authors | J. T. Kindt, S. Tzlil, A. Ben-Shaul and W. M. Gelbart |
| Journal Title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| Volume | 98 |
| Issue | 24 |
| Pages | 13671-13674 |
| Date Published | 2001-11 |
| Type of Article | Journal Article |
| Keywords | CONFORMATION, DOUBLE-STRANDED DNA, ESCHERICHIA-COLI, HEADS, lambda, MODEL, ORGANIZATION, STRESS |
| Abstract | We calculate the forces required to package (or, equivalently, acting to eject) DNA into (from) a bacteriophage capsid, as a function of the loaded (ejected) length, under conditions for which the DNA is either self-repelling or self-attracting. Through computer simulation and analytical theory, we find the loading force to increase more than 10-fold (to tens of piconewtons) during the final third of the loading process, correspondingly, the internal pressure drops 10-fold to a few atmospheres (matching the osmotic pressure in the cell) upon ejection of just a small fraction of the phage genome. We also determine an evolution of the arrangement of packaged DNA from toroidal to spool-like structures. |
| URL | http://www.pnas.org/content/98/24/13671.full.pdf |
